Coding

Part:BBa_K5437007:Design

Designed by: Yu-Li He   Group: iGEM24_Formosa   (2024-09-28)


3-hydroxy-3-methyl-glutaryl-coenzyme A (Hmg-CoA) reductase converts Hmg-CoA into mevalonate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 492
    Illegal BglII site found at 2270
    Illegal BglII site found at 2904
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2368
    Illegal AgeI site found at 1573
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1168


Design Notes

Protein originated from eukaryotic organisms, literature research of prokaryotic gene optimization and IDT Codon Optimization service.


Source

NCBI gene search provided sequence for HmgR

References